9 important factors for laboratory microscope imaging

The microscope is already an indispensable experimental instrument in laboratory teaching equipment. It is often used in microbiology experiment classes. How can the microscope image be more accurate and clear? Here are 9 tips for everyone:

1. When using a microscope, it is important that the object being inspected is made more standard. For example, whether the thickness of the slice is too thick, and whether the coverslip conforms to the national standard.

2. The microscope objective lens can be divided into about 6-8 grades according to grade, the most commonly used is the plan achromatic objective lens. If the lens grade is too low, the image quality will be reduced. Therefore, it is recommended to choose the objective lens above the flat field.

3. The aperture of the condenser lens should be as close as possible to the numerical aperture of the objective lens. To get the best image resolution.

4. The focusing system is an important part of the mechanical part, and the focusing accuracy is also very important. For beginners in biology or operators who are new to microscopes, skilled use of focusing systems can quickly find images and focus, improving work efficiency.

5. When observing the slice, the stage often moves X and Y. For example, the double-layer mechanical mobile platform has good accuracy and stability, and can provide good imaging quality when observing microscopic images.

6. Aspheric light collecting mirror can provide very uniform illumination light, and the contrast of the observed microscopic image is excellent.

7. The field of view light bar should be opened to the appropriate position according to the different magnifications of the object to be inspected and the objective lens. For example, if the opening is too large or too small, the brightness of the field of view will be affected.

8. If the lighting system adopts a lighting device with low temperature and high color temperature, the microscopic image with extremely high resolution and sharpness is observed.

9. Using Kola lighting system, can adjust the optical center image. Scientific-grade microscopes use this system to provide high-quality micrographs for microscopy and professional digital microscopic imaging systems.